X-ray crystallography is the pre-eminent technique for obtaining the 3D structures of macromolecules at the level of individual atoms. These structures are central to understanding the detailed mechanisms of biological processes and to discovering novel therapeutics using a structure-based approach. Great advances continue to be made in the methods for determining crystal structures, pushing the frontiers, allowing the pace of structural understanding to increase and making it possible to respond efficiently and rapidly to emerging threats. This course will help to equip the next generation of scientists with a deep understanding of the tools they need to solve challenging structural projects and an appreciation for what the future holds.